The concise review delves into the role of MSC-EVs in tissue regeneration, immunomodulation, and intracellular communication. Highlighting their therapeutic potential, we present an analysis aimed at advancing cell-based therapies. Additionally, we address current challenges in MSCs' culture and expansion and their impact on the quality, quantity, and functionality of EVs production, critical for advancing therapeutic applications. A must-read to navigate the evolving landscape of regenerative medicine.