Cellovations® Primary Cells & more.
PELOBiotech presents its own prime product line Cellovations®. It is based on their heart, experience and knowledge, what researchers need for a successful and accelerated set-up. Cellovations® is the pure essence of the knowledge of a scientist's challenge: optimal and fast handling, best ingredients for reproducible and predictable results.
Cellovations® sells only the best available cells for your studies. Just name the cell you are looking for. Cellovations® has cells from HUVECs to diseased cells to seldom cells. Besides Stem Cells we offer Immune Cells, adherent Primary Cells from human healthy and diseased donors and more. You can use these as a complete kit, together with special culture media plus Coating Solution for optimal and reproducible results.
Cellovations®Customized Cell Solutions
If researchers are looking for a special cell that is not available via Catalog, they use our comprehensive Cell Sourcing Service from healthy and diseased donors (tissue, cells, plasma…). Some cells are available under GMP for the production of ingredients, extracellular vesicles, and more.
Cellovations® Cell Culture Media.
PELOBIOTECH develops and produces special cell culture media under their brand Cellovations®. for human primary cells, stem cells and embryonic stem cells as well as special supplements for tumor stem cells. For starters they offer EASY – The Cellovations® EASY Endothelial Cell
Growth Medium is a complete medium. It is formulated for the optimal growth of endothelial cells out of large vessels (e.g HUVEC, AOEC, PAEC). It has a long shelf life of 12 months.
As one of the key features Cellovations® offers customized solutions with a choice of prime media in R&D, GMP-like and GMP quality. Plus keep an eye on their xeno-free, serum-free and defined solutions.
Cellovations® Cell Culture Tools.
Research with cells depends on viability and shelf life. With the right tools, you will have outstanding results. Choose from our approved Extracellular Matrices, Cold & Cryo Management, Media supplements, Passaging/Dissociation Solutions, ES/iPS/Reprogramming, Safety Tools Like Mycoplasma Testing, or Transfection Tools Some tools are also available in cGMP or GMP.
Facts, References and FAQs about Cellovations.®
- premium cell culture products with the highest standards, developed in Germany.
- combines more than 40 years of lab experience.
- customize your media on request, starting as little as 5 liters.
Ask for more.
Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or HumanAdipose-Derived Stem Cells
Albrecht, F.B.; Schmidt, F.F.;Volz, A.-C.; Kluger, P.J. Gels 2022, 8, 611. https://doi.org/10.3390/ gels8100611
PFKFB4 interacts with FBXO28 to promote HIF-1α signaling in glioblastoma
Phillips, E., Balss, J., Bethke, F. et al. Oncogenesis 11, 57 (2022). https://doi.org/10.1038/s41389-022-00433-3
Nox4 promotes endothelial differentiation through chromatin remodeling
F. Hahner, F. Moll, T. Warwick, D.M. Hebchen, G.K. Buchmann, J. Epah, W. Abplanalp, T. Schader, S. GÃ¼nther, R. Gilsbach, R.P. Brandes, K. SchrÃ¶der, Redox Biology, Volume 55,
2022, 102381, ISSN 2213-2317, https://doi.org/10.1016/j.redox.2022.102381.
An encapsulated fibrin-based bioartificial tissue construct with integrated macrovessels, microchannels, and capillary tubes
Florian Helms, Sarah Zippusch, Jonathan Theilen, Axel Haverich, Mathias Wilhelmi, Ulrike Böer https://doi.org/10.1002/bit.28111
3D Lung-on-Chip Model Based on Biomimetically Microcurved Culture Membranes
Danielle Baptista, Liliana Moreira Teixeira, David Barata, Zeinab Tahmasebi Birgani, Jasia King, Sander van Riet, Thijs Pasman, André A. Poot, Dimitrios Stamatialis, Robbert J. Rottier, Pieter S. Hiemstra, Aurélie Carlier, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, and Roman Truckenmüller ACS Biomaterials Science & Engineering 2022 8 (6), 2684-2699 DOI: 10.1021/acsbiomaterials.1c01463
A Print-and-Fuse Strategy for Sacrificial Filaments Enables Biomimetically Structured Perfusable Microvascular Networks with Functional Endothelium Inside 3D Hydrogels
Matthias Ryma, Hatice Genç, Ali Nadernezhad, Ilona Paulus, Dominik Schneidereit, Oliver Friedrich, Kristina Andelovic, Stefan Lyer, Christoph Alexiou, Iwona Cicha, Jürgen Groll https://doi.org/10.1002/adma.202200653
Spassov, S.G.; Faller, S.; Goeft, A.; Von Itter, M.-N.A.; Birkigt, A.; Meyerhoefer, P.; Ihle, A.; Seiler, R.; Schumann, S.; Hoetzel, A. Antioxidants 2022, 11, 1001. https://doi.org/10.3390/antiox11051001
How to store cryo-preserved primary cells?
Cellovations® primary cells shipped on dry ice have to be stored in a liquid nitrogen tank immediately on receipt or have to be thawed and used immediately. If needed we send cells also using a dry shipper. Please contact us for further information.
- We provide a wide variety of cells isolated which can be broadly grouped into the following types:
- Fibroblasts: Cells responsible for producing the extracellular matrix of connective tissue. Additionally, extracellular matrix samples from both healthy and diseased donors with conditions such as fibrosis are also available for purchase which are derived from lung and liver tissues.
- Keratinocytes: Cells that make up the majority of the epidermis (outermost layer of the skin) and produce the protein keratin. Keratinocytes are the primary cells found in the epidermis of the skin and play a crucial role in providing a protective barrier against environmental stressors.
- Smooth muscle cells: Cells found in the walls of organs and blood vessels that contract and relax to control their function.
- Epithelial cells: Cells that line the surfaces of the body and internal organs, serving a protective barrier function.
- Pericytes: Cells located along the walls of small blood vessels, regulating blood flow and playing a role in tissue repair.
- Mesenchymal stoma (stem)cells: Cells that can differentiate into different types of cells, such as bone, cartilage, and muscle cells, and are involved in tissue repair and regeneration.
- Astrocytes: Star-shaped cells that provide structural and metabolic support to neurons in the central nervous system.
- Microglia: Cells in the central nervous system that play a role in immune defense and clearing away damaged cells.
- Endothelial cells: Cells lining the inner surface of blood vessels and playing a key role in blood circulation, these can also be HUVECs which are harvested from the umbilical vein.
- Liver stellate cells: Cells in the liver involved in the storage and release of Vitamin A, and producing extracellular matrix proteins.
- Kupffer Cells: Kupffer cells are specialized macrophages that reside in the liver and play a critical role in removing foreign substances and debris from the bloodstream.
- Neuronal cells: Nerve cells that transmit electrical and chemical signals in the brain and nervous system.
- Tenocytes: Cells that make up tendons and play a role in connecting muscles to bones.
- Hepatocytes: a liver cell that performs various metabolic, synthetic, and secretory functions essential for the body's overall health and well-being.
- Melanocytes: Cells that produce the pigment melanin, responsible for skin, hair, and eye color.
- Myocytes: Muscle cells that can contract and generate force to produce movement.
- Podocytes: Specialized cells in the kidneys that form a barrier to prevent proteins from being filtered out of the blood.
- Stromal vascular fraction: the Stromal Vascular Fraction (SVF) is a mixture of cells and growth factors isolated from adipose tissue with potential therapeutic applications, including the high concentration of mesenchymal stem cells with the ability to self-renew and differentiate into various cell types.
Which media is the best for my research?
To determine if a media is suitable for your research, you need to consider several factors:
- Cell type: Different cells require different types of media. Make sure the media you choose is specifically designed for the cell type you are using.
- Nutrient requirements: Different cells have different nutrient requirements, so the media should contain the necessary nutrients for optimal cell growth and function. Some media may also contain additional supplements or factors to support specific cellular processes.
- pH and osmolarity: The pH and osmolarity of the media should be appropriate for the cell type you are using. Check the recommended pH range and osmolarity of the media to ensure compatibility with your cells.
- Serum vs serum-free: Some cells require serum (usually fetal bovine serum) in the media for optimal growth, while others can grow in serum-free media. Consider whether your cells require serum, and if so, what concentration is optimal.
- Sterility: Make sure the media is sterile to avoid contamination and ensure the accuracy of your results.
We provide media for the maintenance and differentiation of different cell types such as:
How can I thaw and plate primary cells?
Please check out the primary cell instructions for details as thawing and plating might be different from cell type to cell type.
- Place the vial of frozen cells in a 37°C water bath for 1 minute with a gentle swirling of the sample until only a little bit of ice is visible.
- Ensure that the vial is not completely submerged and that water does not enter the vial.
- To sterilize, take out the vial from the water bath and clean it with 70% isopropanol or ethanol. Alternatively, for a more consistent and reproducible thawing process, you can use the ThawStar.
- Open the vial and gently pipette the cell suspension up and down to evenly suspend cells.
- Pipette the cells into 10 ml of full endothelial cell media in a 15 ml tube and spin the cells down at 200 x g for 5 minutes.
- Discard the supernatant and resuspend the cells gently with 10ml full endothelial cell media and transfer ALL cells into 1-2 T25 flasks and culture the cells at 37° C in a CO2 incubator.
How do I passage primary cells?
Passaging primary cells involves the transfer of cells from one culture vessel to another to maintain the cells in a healthy and growing state. Here are the general steps for passaging primary cells:
- Prepare the culture vessel: Coat a new culture vessel with appropriate extracellular matrix proteins or attachment factors to promote cell adhesion.
- Prepare the dissociation solution: Prepare a dissociation solution appropriate for the cell type. Trypsin-EDTA* is a common dissociation solution for many cell types, but some cells may require other enzymes or buffers.
*For the detachment of the cells Trypsin / EDTA, TrypLE, or Accutase can be used. Below please find a summarized overview of their properties.
Accutase: (Cat# PB-PAACCUTASE) is a mixture of proteolytic and collagenolytic enzymes isolated from crustacean; is mammary component free; for some cell types no need for inactivation or removal during passaging; is very gentle for cells, thus most of the surface proteins are intact after passaging; has to be stored at 4°C; Accutase is inactivated automatically after 1 h at 37°C.
Trypsin / EDTA: (PB-900028 – Passage Pack) is a solution of proteolytic enzymes containing Trypsin, Chymotrypsin, and Elastase isolated from the porcine pancreas that shows lot-to-lot variability in activity; is commonly used in cell culture; is available from different suppliers; has to be inactivated (e.g. FCS alone, FCS supplemented medium or trypsin inhibitor from soybean); compared with Accutase more cell culture experience for the user is necessary because cells can be irreversibly damaged by too high trypsin concentrations or too long incubation times.
- Aspirate the culture media: Remove the old culture media from the primary cell culture.
- Wash cells with PBS: Rinse the cells with phosphate-buffered saline (PBS) to remove any residual media and serum components.
- Add dissociation solution: Add enough dissociation solution to the culture vessel to cover the cells.
- Incubate cells: Incubate the cells for an appropriate period at 37°C to allow the dissociation solution to break the cell-cell and cell-substrate adhesions and release the cells.
- Neutralize dissociation solution: Add an equal volume of complete media (containing animal-free and defined serum replacement/serum/soybean trypsin inhibitor and L-Glutamine) to neutralize the dissociation solution and inactivate any residual trypsin or other enzymes.
- Collect cells: Collect the cells by pipetting the solution up and down several times to break up any cell clumps.
- Count cells: Count the cells using a hemocytometer or an automated cell counter to determine the cell concentration.
- Plate cells: Plate the cells into the new culture vessel, adding appropriate media to achieve the desired cell density.
- Incubate cells: Incubate the cells at 37°C with appropriate culture conditions.
It is important to optimize the cell density, media composition, and culture conditions for each specific cell type to ensure optimal growth and survival of the primary cells after passaging.
What is the Speed Coating Solution?
Our Speed Coating Solution is a ready-to-use solution to increase cell attachment. It is a mixture of animal ECMs and it shortens significantly the time of trypsinization of cells during passaging. Speed Coating Solution can be used for endothelial cells, epithelial cells, smooth muscle cells, and fibroblasts.
What are the risks of cell line contamination?
Cell line contamination can result in changes in cell growth, gene expression, and protein synthesis, leading to inaccurate experimental results and potentially affecting downstream applications, misinterpretation of experimental results, and can lead to cross-contamination of other cell lines. Proper aseptic techniques and regular monitoring are essential to prevent contamination. Mycoplasma contamination is a common form of cell culture contamination caused by a group of small, bacteria-like microorganisms that can infect cell cultures and compromise experimental results. 15-35% of all cell lines are contaminated with mycoplasma
Click here to prevent mycoplasma contamination:
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How can mycoplasma contamination be prevented and handled?
Prevention of mycoplasma contamination involves maintaining aseptic techniques, using sterile media and reagents, and regular monitoring of cell cultures. If contamination is suspected or confirmed, immediate action should be taken to isolate the contaminated cultures, decontaminate the laboratory and equipment, and re-establish a mycoplasma-free cell culture system. Additionally, it is important to verify that any contaminated experiments or data are identified and excluded from the analysis.
What are the challenges of handling stem cells in cell culture?
Stem cells require specialized culture conditions, including the use of specific media, coatings, and substrates. Additionally, like all cell lines, they are sensitive to environmental factors such as pH, temperature, and osmolarity, making it crucial to maintain a sterile environment and use aseptic techniques to prevent contamination. The identity and purity of stem cells can be verified using various methods, such as immunocytochemistry, flow cytometry, and gene expression analysis. It is important to use validated markers and protocols to ensure accurate identification and characterization of the cells.
Activin-A, BDNF, BMP-2, BMP-7, CCM-2, EGF, EGFR, FGF-2, FGFR, GM-CSF, gremlin-1, HGF, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-10, LIF, PDGF-AA, PDGF-BB, PIGF-1, SCF, and TIE-2 are all growth factors or cytokines that can be added to the cell culture medium to modulate various cellular processes such as proliferation, differentiation, and survival. We provide these and more protein and cytokines in animal-free forms as well!
What assays can be done with mesenchymal stem cells?
- Colony-forming unit (CFU) assay: used to quantify the self-renewal capacity of mesenchymal stem cells (MSCs).
- Differentiation assays: used to evaluate the differentiation potential of MSCs into various cell lineages, such as osteogenic, adipogenic, and chondrogenic lineages.
- Proliferation assays: used to monitor cell growth and proliferation of MSCs in culture.
What are the applications of mesenchymal stem cells?
- Regenerative medicine: MSCs have the potential to differentiate into various cell types and can be used in tissue engineering and regenerative medicine applications to repair and regenerate damaged tissues.
- Immunomodulation: MSCs have been shown to modulate the immune response, making them a potential therapeutic option for autoimmune and inflammatory diseases.
- Drug discovery and toxicology: MSCs can be used as a model system to test drug efficacy and toxicity in vitro.
- Gene therapy: MSCs can be genetically modified to express therapeutic genes and used as a delivery system for gene therapy.
What is a coculture growth medium and what can I use it for?
Co-culture growth medium is a type of cell culture medium that is designed to support the growth and survival of two or more different types of cells in the same culture vessel. This type of culture is called "co-culture".
Co-culture is used to study the interactions between different types of cells, such as between immune cells and cancer cells or between neurons and glial cells. In these cultures, different cell types are grown together in the same culture vessel to mimic the in vivo microenvironment and study their interactions.
Coculture growth medium typically contains a mixture of nutrients, growth factors, and other components that are optimized to support the growth and survival of both cell types. The composition of the medium can vary depending on the specific cell types being cultured and their nutritional requirements.
In some cases, the coculture growth medium may also contain supplements that are designed to promote or inhibit specific interactions between the different cell types. For example, certain cytokines or growth factors may be added to promote the differentiation of stem cells into a specific cell type or to induce immune cell activation.
What are the applications of ALK5 inhibitors?
ALK5 (Activin receptor-like kinase 5) inhibitors are a class of drugs that block the activity of ALK5, a type of receptor protein involved in a signaling pathway called the TGF-beta pathway.
In particular, ALK5 inhibitors can be used to enhance the expansion of mesenchymal stem cells (MSCs), which are a type of adult stem cell that can differentiate into a variety of cell types, including bone, cartilage, and fat cells.
In culture, ALK5 inhibitors can maintain MSCs in an undifferentiated state and promote their proliferation. This makes them a valuable tool for researchers who want to generate large numbers of stem cells for use in tissue engineering or regenerative medicine applications.
Additionally, ALK5 inhibitors have been shown to enhance the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). iPSCs are cells that have been reprogrammed to an embryonic-like state and can differentiate into any cell type in the body. The use of ALK5 inhibitors during the reprogramming process can increase the efficiency and speed of iPSC generation.
ALK5 inhibitors have shown promise in the treatment of fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). By blocking ALK5 activity, these drugs can reduce the accumulation of excess extracellular matrix (ECM) proteins that contribute to tissue scarring and fibrosis. Additionally, ALK5 inhibitors are being investigated as potential cancer therapies, as the TGF-beta pathway can promote tumor growth and metastasis. Inhibition of this pathway using ALK5 inhibitors may help to slow or halt cancer progression.
What is antibiotic resistance?
Antibiotic resistance is the ability of bacteria or other microorganisms to resist the effects of antibiotics, making infections difficult or impossible to treat. This can happen when bacteria mutate or acquire resistance genes, which allow them to survive exposure to antibiotics that would normally kill or inhibit their growth.
Amphotericin is an antibiotic that is commonly used in cell culture to prevent contamination from bacteria and fungi, respectively. This antibiotic can be effective in preventing contamination and reducing the risk of antibiotic resistance, as they have different mechanisms of action than penicillin and streptomycin.
Penicillin and streptomycin are commonly used antibiotics in cell culture, but they have been associated with the development of antibiotic resistance in bacteria. By using alternative antibiotics such as amphotericin instead, we can reduce the selective pressure on bacteria to develop resistance to penicillin and streptomycin.
What is the need for L-Glutamine as a supplement in cell culture?
L-Glutamine is an important supplement in cell culture media as it is an essential amino acid required for cell growth and proliferation. It is a key source of energy and nitrogen for cells and is involved in the biosynthesis of proteins, nucleotides, and other important biomolecules.
Most cells can synthesize L-Glutamine, but they require more than can be produced in vitro, especially during rapid growth phases. Therefore, the addition of L-Glutamine to cell culture media ensures that cells have enough of this essential nutrient to support optimal growth and metabolism.
L-Glutamine is relatively unstable in aqueous solutions and can spontaneously break down to form toxic by-products, so it is often added to cell culture media as a stable dipeptide, L-Alanyl-L-Glutamine or L-Glutamine-MAXIMUM, to prevent degradation and enhance cell viability.