PELOBiotech

Cellovations® primary cells shipped on dry ice have to be stored in a liquid nitrogen tank immediately on receipt or have to be thawed and used immediately. If needed we send cells also using a dry shipper. Please contact us for further information.

• We provide a wide variety of cells isolated which can be broadly grouped into the following types:

• Fibroblasts: Cells responsible for producing the extracellular matrix of connective tissue. Additionally, extracellular matrix samples from both healthy and diseased donors with conditions such as fibrosis are also available for purchase which are derived from lung and liver tissues.
• Keratinocytes: Cells that make up the majority of the epidermis (outermost layer of the skin) and produce the protein keratin. Keratinocytes are the primary cells found in the epidermis of the skin and play a crucial role in providing a protective barrier against environmental stressors.
• Smooth muscle cells: Cells found in the walls of organs and blood vessels that contract and relax to control their function.
• Epithelial cells: Cells that line the surfaces of the body and internal organs, serving a protective barrier function.
• Pericytes: Cells located along the walls of small blood vessels, regulating blood flow and playing a role in tissue repair.
• Mesenchymal stoma (stem)cells: Cells that can differentiate into different types of cells, such as bone, cartilage, and muscle cells, and are involved in tissue repair and regeneration.
• Astrocytes: Star-shaped cells that provide structural and metabolic support to neurons in the central nervous system.
• Microglia: Cells in the central nervous system that play a role in immune defense and clearing away damaged cells.
• Endothelial cells: Cells lining the inner surface of blood vessels and playing a key role in blood circulation, these can also be HUVECs which are harvested from the umbilical vein.
• Liver stellate cells: Cells in the liver involved in the storage and release of Vitamin A, and producing extracellular matrix proteins.
• Kupffer Cells: Kupffer cells are specialized macrophages that reside in the liver and play a critical role in removing foreign substances and debris from the bloodstream.
• Neuronal cells: Nerve cells that transmit electrical and chemical signals in the brain and nervous system.
• Tenocytes: Cells that make up tendons and play a role in connecting muscles to bones.
• Hepatocytes: a liver cell that performs various metabolic, synthetic, and secretory functions essential for the body's overall health and well-being.
• Melanocytes: Cells that produce the pigment melanin, responsible for skin, hair, and eye color.
• Myocytes: Muscle cells that can contract and generate force to produce movement.
• Podocytes: Specialized cells in the kidneys that form a barrier to prevent proteins from being filtered out of the blood.
• Stromal vascular fraction: the Stromal Vascular Fraction (SVF) is a mixture of cells and growth factors isolated from adipose tissue with potential therapeutic applications, including the high concentration of mesenchymal stem cells with the ability to self-renew and differentiate into various cell types.

To determine if a media is suitable for your research, you need to consider several factors:

1. Cell type: Different cells require different types of media. Make sure the media you choose is specifically designed for the cell type you are using.
2. Nutrient requirements: Different cells have different nutrient requirements, so the media should contain the necessary nutrients for optimal cell growth and function. Some media may also contain additional supplements or factors to support specific cellular processes.
3. pH and osmolarity: The pH and osmolarity of the media should be appropriate for the cell type you are using. Check the recommended pH range and osmolarity of the media to ensure compatibility with your cells.
4. Serum vs serum-free: Some cells require serum (usually fetal bovine serum) in the media for optimal growth, while others can grow in serum-free media. Consider whether your cells require serum, and if so, what concentration is optimal.
5. Sterility: Make sure the media is sterile to avoid contamination and ensure the accuracy of your results.

We provide media for the maintenance and differentiation of different cell types such as:

Adipocytes

Tenocytes

Stellate cells

Smooth muscle cells

Skeletal muscle cells

Pericytes

Neural cells

Myocytes

Stromal/mesenchymal cells

Melanocytes

iPSC cells

Hepatocytes

Fibroblasts

Epithelial cells

Endothelial cells

Please check out the primary cell instructions for details as thawing and plating might be different from cell type to cell type.

1. Place the vial of frozen cells in a 37°C water bath for 1 minute with a gentle swirling of the sample until only a little bit of ice is visible.
2. Ensure that the vial is not completely submerged and that water does not enter the vial.
3. To sterilize, take out the vial from the water bath and clean it with 70% isopropanol or ethanol. Alternatively, for a more consistent and reproducible thawing process, you can use the ThawStar.
4. Open the vial and gently pipette the cell suspension up and down to evenly suspend cells.
5. Pipette the cells into 10 ml of full endothelial cell media in a 15 ml tube and spin the cells down at 200 x g for 5 minutes.
6. Discard the supernatant and resuspend the cells gently with 10ml full endothelial cell media and transfer ALL cells into 1-2 T25 flasks and culture the cells at 37° C in a CO2 incubator.

Our Speed Coating Solution is a ready-to-use solution to increase cell attachment. It is a mixture of animal ECMs and it shortens significantly the time of trypsinization of cells during passaging. Speed Coating Solution can be used for endothelial cells, epithelial cells, smooth muscle cells, and fibroblasts.

Cell line contamination can result in changes in cell growth, gene expression, and protein synthesis, leading to inaccurate experimental results and potentially affecting downstream applications, misinterpretation of experimental results, and can lead to cross-contamination of other cell lines. Proper aseptic techniques and regular monitoring are essential to prevent contamination. Mycoplasma contamination is a common form of cell culture contamination caused by a group of small, bacteria-like microorganisms that can infect cell cultures and compromise experimental results. 15-35% of all cell lines are contaminated with mycoplasma

Click here to prevent mycoplasma contamination:

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Prevention of mycoplasma contamination involves maintaining aseptic techniques, using sterile media and reagents, and regular monitoring of cell cultures. If contamination is suspected or confirmed, immediate action should be taken to isolate the contaminated cultures, decontaminate the laboratory and equipment, and re-establish a mycoplasma-free cell culture system. Additionally, it is important to verify that any contaminated experiments or data are identified and excluded from the analysis.

Stem cells require specialized culture conditions, including the use of specific media, coatings, and substrates. Additionally, like all cell lines, they are sensitive to environmental factors such as pH, temperature, and osmolarity, making it crucial to maintain a sterile environment and use aseptic techniques to prevent contamination. The identity and purity of stem cells can be verified using various methods, such as immunocytochemistry, flow cytometry, and gene expression analysis. It is important to use validated markers and protocols to ensure accurate identification and characterization of the cells.

Activin-A, BDNF, BMP-2, BMP-7, CCM-2, EGF, EGFR, FGF-2, FGFR, GM-CSF, gremlin-1, HGF, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-10, LIF, PDGF-AA, PDGF-BB, PIGF-1, SCF, and TIE-2 are all growth factors or cytokines that can be added to the cell culture medium to modulate various cellular processes such as proliferation, differentiation, and survival. We provide these and more protein and cytokines in animal-free forms as well!

• Colony-forming unit (CFU) assay: used to quantify the self-renewal capacity of mesenchymal stem cells (MSCs).
• Differentiation assays: used to evaluate the differentiation potential of MSCs into various cell lineages, such as osteogenic, adipogenic, and chondrogenic lineages.
• Proliferation assays: used to monitor cell growth and proliferation of MSCs in culture.

• Regenerative medicine: MSCs have the potential to differentiate into various cell types and can be used in tissue engineering and regenerative medicine applications to repair and regenerate damaged tissues.
• Immunomodulation: MSCs have been shown to modulate the immune response, making them a potential therapeutic option for autoimmune and inflammatory diseases.
• Drug discovery and toxicology: MSCs can be used as a model system to test drug efficacy and toxicity in vitro.
• Gene therapy: MSCs can be genetically modified to express therapeutic genes and used as a delivery system for gene therapy.

Co-culture growth medium is a type of cell culture medium that is designed to support the growth and survival of two or more different types of cells in the same culture vessel. This type of culture is called "co-culture".

Co-culture is used to study the interactions between different types of cells, such as between immune cells and cancer cells or between neurons and glial cells. In these cultures, different cell types are grown together in the same culture vessel to mimic the in vivo microenvironment and study their interactions.

Coculture growth medium typically contains a mixture of nutrients, growth factors, and other components that are optimized to support the growth and survival of both cell types. The composition of the medium can vary depending on the specific cell types being cultured and their nutritional requirements.

In some cases, the coculture growth medium may also contain supplements that are designed to promote or inhibit specific interactions between the different cell types. For example, certain cytokines or growth factors may be added to promote the differentiation of stem cells into a specific cell type or to induce immune cell activation.

ALK5 (Activin receptor-like kinase 5) inhibitors are a class of drugs that block the activity of ALK5, a type of receptor protein involved in a signaling pathway called the TGF-beta pathway.

In particular, ALK5 inhibitors can be used to enhance the expansion of mesenchymal stem cells (MSCs), which are a type of adult stem cell that can differentiate into a variety of cell types, including bone, cartilage, and fat cells.

In culture, ALK5 inhibitors can maintain MSCs in an undifferentiated state and promote their proliferation. This makes them a valuable tool for researchers who want to generate large numbers of stem cells for use in tissue engineering or regenerative medicine applications.

Additionally, ALK5 inhibitors have been shown to enhance the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). iPSCs are cells that have been reprogrammed to an embryonic-like state and can differentiate into any cell type in the body. The use of ALK5 inhibitors during the reprogramming process can increase the efficiency and speed of iPSC generation.

ALK5 inhibitors have shown promise in the treatment of fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). By blocking ALK5 activity, these drugs can reduce the accumulation of excess extracellular matrix (ECM) proteins that contribute to tissue scarring and fibrosis. Additionally, ALK5 inhibitors are being investigated as potential cancer therapies, as the TGF-beta pathway can promote tumor growth and metastasis. Inhibition of this pathway using ALK5 inhibitors may help to slow or halt cancer progression.

Antibiotic resistance is the ability of bacteria or other microorganisms to resist the effects of antibiotics, making infections difficult or impossible to treat. This can happen when bacteria mutate or acquire resistance genes, which allow them to survive exposure to antibiotics that would normally kill or inhibit their growth.

Amphotericin is an antibiotic that is commonly used in cell culture to prevent contamination from bacteria and fungi, respectively. This antibiotic can be effective in preventing contamination and reducing the risk of antibiotic resistance, as they have different mechanisms of action than penicillin and streptomycin.

Penicillin and streptomycin are commonly used antibiotics in cell culture, but they have been associated with the development of antibiotic resistance in bacteria. By using alternative antibiotics such as amphotericin instead, we can reduce the selective pressure on bacteria to develop resistance to penicillin and streptomycin.