Assays offered by PELOBiotech.

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We offer a lot in the field of ​​Assays, but what else does PELOBiotech offer?

We are more than competent4cells – and that also in the field of Assays. From quality products to fast delivery we are your competent cell culture experts.

  • We are the qualified, solution-oriented experts you have been looking for.
  • We provide you with one of the best cell portfolios for your research needs.
  • We fill the gap and build a bridge between innovative research and the special requests of the clinical sector.
  • Get proficient recommendations and info first hand.

Take the opportunity and trust our expertise and rely on our products in the field of Assays.

Assays are a great tool.

We offer a lot of innovative and standard assays to make your life easier. Assays are great to test a variety of different features and to get more information about one specific detail for your research.

Because we know your time is valuable, we offer a lot of innovative and standard assays to make your life easier with their easy-to-handle systems.

You may know in very early stages of your project which way may be the best to choose. At crucial points you get the right hints to start over and get your protocols standardized. We have standardized assays like ELISA and functional assays like Cancer Stem Cell assays for Leukemic cells, Colony Forming assays (CFUs) or Angiogenesis tracing kits.

Our innovative and standard Assays.

We have the right test kits, with instructions and reagents. What more do you need?

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Assay Kits

Such as 3D Cell Invasion Assay, Angiogenesis, Apoptose, Cell Adhesion, Cell Characterization, Stem Cell Differentiation (CFC), Cell Proliferation, Cell Viability, ELISA, Cell Purity for Chimerism Analysis, Mycoplasma Detection, Trace Metal Assay Kits.

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„The goal of our company is to provide you with the best cell portfolio for your research needs and to give you customized solutions to your specific research problems.“

Dr. Peter Frost, CEO PELOBIOTECH GmbH

More Than Competence4Cells.

Life Science research
with PELOBiotech.

Cell Culture offer

More than 20,000 high-end Cell Culture products.

Products

Broad portfolio in the field of cells, media and tools.

Years of experience

CEOs have around 35 years of experience in their field.

Certifications

„INNOVATIVE Through Research”.

Number of suppliers

Over 30 global suppliers.

Number of distributors

2 distributors, based in Japan and Switzerland.

Location of own brand development

Germany.

Location of own brand production

Germany.

Daily Business

Cell sourcing and customized cell isolation from healthy and / or diseased donors.

Services

Contract research, product development & production, 3D cell culture Services, Compound testing, reliable assays, Knowledge transfer.

Spoken Languages

German, English and French.

Assays: Get the Details, Easy.

Standard meets innovation. Find out more about our test kits.

3D Cell Invasion Assay

GradientEZ is a qualitative cell invasion and migration assay which enables cells to migrate in three dimensions as they do in vivo.

Application:

  • Cell migration assays
  • Cell invasion assays
  • Chemo-invasion assays
  • Chemotaxis assays
  • Metastatic potential

Angiogenesis

The human AngioTracing Kit incorporates GFP-tagged human endothelial cells from variable tissues that are co-cultured with RFP-tagged human mesenchymal supporting cells in a specially designed medium. The systems will form a network of anastomosing tubules that closely resembles the capillary bed found in vivo after 1-2 weeks.

The Angiogenesis Tracing Kits are designed to content:

  • 1 vial of pre-mixed and tagged human primary cells (endothelial cells & mesenchymal stem cells)
  • 1 × 14 ml Speed Coating Solution
  • 1 × 500 ml 1 × 500ml of Endo-Growth Medium (ready-to-use)

We offer the following kits (24 well-format):

  • PB-cAP-08 In vitro HUVECs Angiogenesis Tracing Kit
  • PB-cAP-12 In vitro Human Brain Microvascular Angiogenesis Tracing Kit
  • PB-cAP-13 In vitro Human Pancreatic Microvascular Endothelial Angiogenesis Tracing Kit
  • PB-cAP-14 In vitro Human Glomerular Microvascular Angiogenesis Tracing Kit
  • PB-cAP-15 In vitro Human Lymphangiogenesis Tracing Kit
  • PB-cAP-16 In vitro Human Retina Microvascular Angiogenesis Tracing Kit

Apoptose

Apoptosis is an extreme of cell death and is characterized by several biochemical features e.g. cell shrinkage, membrane blebbing, chromosome condensation, nuclear fragmentation and DNA laddering.

We offer different assays to detect induced or ongoing apoptosis via Annexin, Caspase, Cathepsin D or DNA strand breaks (TUNEL).

Cell Adhesion

The MicroMatrix matrix (ECM) array enables rapid screening of 36 different combinations of extracellular matrix proteins (ECMPs) in a single experiment, requiring only a limited population of cells. Each of the 36 ECM conditions on a MicroMatrix array consists of one to several matrix proteins, spotted onto a thin hydrogel layer. Cells will attach only to the ECM spots with greater cell density and superior morphology being observed in the matrix conditions for which the cells have an affinity. Once identified on the MicroMatrix array the ECM conditions that support your cell's attachment, morphology and other desired outcomes can be further investigated in a chambered slide system or ordered as an optimized solution to coat labware.

The assays can be used also to investigate the influence of the microenvironment of stem cell differentiation. MicroMatrix 36 Array - PB-MM012011

Cell Characterization

Cell based Elisa are used to characterize human primary cell differentiation / un-differentiation (adipocytes, chondrocytes and osteoblasts), adult stem cell differentiation (MSC) or Cancer Stem Cells (Breast CSCs and Pancreatic CSCs).

Stem Cell Differentiation (CFC)

About Colony Forming Cell Assays. Hematopoietic progenitors with lineage-restricted (less primitive) or multi-lineage (more primitive) potential form morphologically distinct colonies when grown in a suitable semi-solid medium containing appropriate growth factors and other supplements. To form the specific colony type that characterizes its potential, a progenitor cell must both proliferate and differentiate within the supportive matrix.

Colony-forming cell (CFC) assays therefore have great utility in the identification and enumeration of the different types of functional progenitors in hematopoietic tissues (e.g. bone marrow, cord blood, fetal liver) and manipulated cell populations (e.g. purified, expanded, frozen/thawed, drug treated, etc.).

For a variety of reasons, methylcellulose has become the matrix of choice for semi-solid media for CFC assays. Pre-screening and qualification of the various components of methylcellulose-based media allow for media products that are highly reproducible in their ability to support vigorous and robust growth of the various types of progenitors.

ColonyGEL™ meets the highest standards of methylcellulose-based media for hematopoietic colony-forming cell assays.

Cell Proliferation

We offer a proliferation assay based on XTT which gives you results in 2-5 hours. You need just an ELISA reader and the complete assay is performed in a microtiter plate.
MTT and WST-1 assays are available, too.

Cell Viability

Cell viability /vitality assays give you the number of healthy cells in your culture. We offer assays based on MTT or WST-1.

ELISA

We offer ELISA kits for different topics e.g.

  • Metabolic Syndrom - Diabetes
  • Hypertension & Stroke
  • Oxidative Stress-Sepsis
  • Cancer Biomarker
  • BPA
  • Drug Metabolism (P450-GST)
  • Cardio Tox
  • Cytokines, Growth factors & Chemokines
  • ECM Proteins
  • other

Cell Purity For Chimerism Analysis

Assess the purity of sorted T-cells, B-cells, or Myeloid cells directly in the genomic DNA sample for lineage-specific chimerism analysis.

Benefits:

  • Save cells; stop performing flow cytometry
  • Use only 2 ng of DNA (less than 1,000 cells!)
  • Can be performed on frozen DNA, even years after cell sorting

Mycoplasma Detection

Mycoplasma Total Solution

  • Mycoplasma Detection
  • Mycoplasma Elimination
  • Mycoplasma Prevention
  • Mycoplasma-free cell freezing medium

Trace Metal Assay Kits

Measure trace metals by microplate reader within 10 minutes! The kits which we offer do not contain any hazardous components like cyanide or azide as a preservative.
Samples: e.g. serum, plasma, urine, salvias, cell lysates, tissue extracts, hair extracts and water.

  • Fe
  • Cu
  • Zn
  • Mg
  • Ca
  • Li (serum and plasma; heparin lithium samples cannot be used!)

Every research has its challenge. Where is yours?

We’re proud to assist scientists and researchers like you!

10/10 would recommend

(5.0)

I reached out to PELOBiotech to get a specific cell line + the optimal cell culture medium for our laboratory and Dr. Arista was the one assisting me in this whole process. Their response time was incredibly fast, their customer services more than satisfactory and their planning ahead made the whole order/delivery process of the items (they had to be imported from USA) smooth, efficient and once again, very fast. Pleasantly surprised that we can still find this type of customer service/approach nowadays.

Very pleased with the stable growth performance of my cells

(5.0)

"I´ve been very pleased with the stable growth performance of my cells with the new medium Endothelial Growth medium EASY from Cellovations. I will continue using it for my experiments."

The service was outstanding

(5.0)

"PELOBiotech’s media have contributed to optimal experimental conditions for my endothelial cells and the service, especially the scientific support, was outstanding.”

Epithelial cells for 3D culture are easy to handle

(5.0)

“I used human primary pancreatic epithelial cells for 3D culture and immunostaining. They are easy to handle and I could expand them for 3 passages. Overall these cells are useful source for study pancreatic epithelium in humans, thought with limited passage number and modification.”

With that medium our cells grow perfectly

(5.0)

"I’m using PELOBiotech’s Skeletal Muscle Cell Growth Medium for my own isolated primary cells from clinics. With that medium our cells grow perfectly well and we love the reliability of the medium and the service of the company."