The 3-in-1 Plate is a versatile hydrogel insert that simplifies spheroid/organoid formation, ECM integration, and drug testing, all in a single platform. It enables disturbance-free solution exchanges and in situ IHC analysis, reducing the risk of losing spheroids. The microwells of the insert ensure uniform models and require fewer cells to form organoids, eliminating the need for passaging. It is suitable for a wide range of applications, including coculture, invasion studies, drug screening, assays, and imaging. Compared to traditional 2D cell culture, 3D cell culture in the 3-in-1 Plate better mimics the in vivo tissue microenvironment, resulting in more accurate gene expression profiles and increased cell communication, proliferation, and survival rates. The platform is compatible with in-situ immunostaining, fluorescence microscopy, and downstream cytometry and omics analysis.

• The 3-in-1 Plate is a hydrogel insert that fits within each well of a six-well plate
• It combines spheroid/organoid formation, extracellular matrix (ECM) integration, and drug testing all in one platform
• Ideal for researchers looking to embed spheroids in an ECM or conduct drug screening on complex models
• Eliminates the need for transferring models from one plate to another, ensuring disturbance-free solution exchanges
• Allows in situ IHC analysis and slicing of spheroids directly within the microwells of the 3-in-1 Plate, preserving the delicate morphology of models
• Provides highly uniform spheroids and perfect data consistency due to the geometry of the microwells
• Requires fewer cells to form organoids, eliminating the need for passaging
• Applications include coculture, invasion, drug screening, assays, and imaging
• 2D cell cultures have significant limitations and are not an accurate representation of in vivo tissue
• 3D cell culture is an increasingly popular research tool that mimics the native microenvironment from which cells originated and provides more accurate gene expression profiles
• The 3-in-1 Plate allows for IHC and H&E treatment directly in the platform without removing spheroids and can be embedded in paraffin and sliced without further manipulation
• The platform is compatible with in-situ immunostaining and fluorescence microscopy, and spheroids can be easily dissociated on the plate for downstream cytometry analysis or used for omics analysis.