PELOProductNews: Novel Dye for Metabolism Research now available - LipiDye-M

From CB 08. November 2022 3
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Lipids are the basic components of cells, forming membrane structures and serving as signaling molecules. Fatty acids (FAs) are the tiniest and most necessary building blocks of lipids. FAs are not only biochemically synthesized in the cell, but also taken up from the extracellular space by FA transporters.

Regulation by enzymes

Intracellular FAs are metabolically converted into various lipids, including acyl-CoA, phospholipids, glycolipids, diacylglycerols (DAGs), and triacylglycerols (TAGs), and also degraded mainly by the mitochondrial fatty acid beta-oxidation (FAO) pathway for energy production and by lipid droplet-selective autophagy, lipophagy. Lipid metabolism is tightly regulated by various enzymes in the cell, and its abnormal regulation leads to diseases such as obesity, diabetes, etc.

Fluorescent colors in different intracellular environments

LipiDye-M is a novel fluorescent dye-labeled C12 fatty acid with a negative solvatochromic dye called 3a-azapyren-4-one (AP). LipiDye-M ( was originally developed by Dr. Shigehiro Yamaguchi and Dr. Masayasu Taki of Nagoya University. LipiDye-M is a mimic of FA that is approximately the same length as C18-FA, such as stearic acid and oleic acid. The AP dye recognizes the polarity of the environment and changes its absorption and fluorescence spectrum . Although AP dye
shows red fluorescence at lower polarity, e.g. in hydrophobic oil, it emits green fluorescence at higher polarity, e.g. in aqueous solution. Due to the solvatochromic property of AP, LipiDye-M can exhibit its fluorescent colors in
different intracellular environments such as cytosol, organelle membranes and lipid droplets.

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