Cell Biologics is an expert in Primary Cell Culture products.
Cell Biologics is one of the main manufacturers of animal and human primary cells. Beside cells and tissues, they also offer a broad portfolio of cell culture media, reagents and cell based assays.
Facts & FAQs about Cell Biologics.
What method is used for the isolation of primary endothelial cells?
FAQ about Custom Primary Endothelial Cell Isolation
The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are isolated with anti-PECAM-1 antibody-conjugated magnetic beads.
What passage number whould they be?
You will receive endothelial cells from passages 2 or 3, depending on cell purity.
How many passages can primary cells be maintained?
Primary endothelial cells can be expanded 3-6 passages by the split ratio of 1:2 under the cell culture conditions specified by Cell Biologics (Recommended Mouse Endothelial Cell Culture Medium: M1168 or M1168CO).
How many cells should you expect to receive from one order?
There are 1-2 million cells/frozen vial provided with each order depending on cell types; 2-4 million cells can be made as per your request.
The cells are frozen at one million cells/ml per vial or per request you can receive proliferating cells in a T75 flask/3 million cells.
Cell Biologics ships frozen cells on dry ice to anywhere in the world. On receipt, frozen cells can be transferred to liquid nitrogen until needed experimental use. Live cell shipment is also available on request.
