Accumol

Accumol PELOBiotech GmbH

Accumol develops genomic purity detection kits and more

Accumol specializes in developing innovative molecular biology reagents for clinical laboratories, with particular expertise in genomic purity detection kits that assess the purity of sorted cell populations (T-cells, B-cells, Myeloid cells, NK cells) directly at the DNA level for chimerism analysis and hematopoietic stem cell transplantation monitoring,

 

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Genomic detection kits

Non-T genomic detection kit


The Non-T Genomic Detection Kit detects contaminating non-T cell DNA in sorted T cell (CD3+ lymphocyte) preparations. The kit exploits the natural deletion of genomic DNA during T-cell receptor (TCR) gene rearrangement, amplifying sequences from the excised region that are absent in mature T cells but present in contaminating cells. Available in both classical PCR and qPCR formats, it requires only 2 ng of DNA (approximately 1,000 cells) and provides ready-to-use pre-filled reaction tubes.

Non-B genomic detection kit


The Non-B Genomic Detection Kit identifies non-B cell DNA contamination in sorted B cell samples by targeting genomic sequences excised during immunoglobulin heavy chain (IGH) gene rearrangement. Like the Non-T kit, it detects DNA from contaminating cells that retain these excised sequences. The kit is provided as pre-aliquoted reaction mix in PCR tubes requiring only template addition before thermocycler loading.

 

Non-myeloid genomic detection kit


The qPCR Non-Myeloid Genomic Detection Kit detects T-cell and B-cell DNA contamination in sorted myeloid cell preparations. This kit enables purity assessment of myeloid populations for lineage-specific chimerism analysis.

MTBN purity assessment kit


The MTBN Purity Assessment Kit detects monocyte, T-cell, B-cell, and neutrophil mRNA in genomic DNA samples from sorted cells. A newer MTBNK kit variant is being developed to additionally assess NK cell purity alongside myeloid cells.

References for Accumol

Clark, J. R., et. al., & United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group. (2015). Monitoring of chimerism following allogeneic haematopoietic stem cell transplantation (HSCT): technical recommendations for the use of short tandem repeat (STR) based techniques, on behalf of the United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group. British Journal of Haematology, 168(1), 26–37. https://doi.org/10.1111/bjh.13073

Lebedeva, T., et al. (2014). Assessment of cell fraction purity by PCR on sequential lineage-specific cell separations for chimerism monitoring [Poster presentation]. Human Immunology, 75(Suppl.), 103. https://doi.org/10.1016/j.humimm.2014.08.218