Thermolysin & Human Islet Isolation: Truth vs. Fiction

Our partner VitaCyte has published a great summary. It´s very helpful. So we like to share with you what their CEO BobMcCarthy wrote:

More human islet isolations have been performed using purified collagenase and thermolysin than any other purified enzyme mixture (1). Several reports have shown the adverse effects when using thermolysin for human islet isolation (2, 3). This blog post reviews the truth and fiction when using thermolysin for human islet isolation. It will also briefly discuss the recent change in thermolysin made by the supplier to an animal origin-free product.

Choice of thermolysin in Liberase HI formulation based on cost, not performance: Cost was the primary factor for use of thermolysin in Liberase™ HI Purified Enzyme Blend. Purified collagenase and neutral protease are required to release islets from pancreatic tissue. Initially, the goal of the Liberase project team was to purify Clostridium histolyticum neutral protease (CHNP) from the same C. histolyticum raw material used as a source of the collagenase. However, this enzyme was difficult to purify and was unstable. The assumption was other neutral proteases would be as effective as CHNP for human islet isolation. Purified thermolysin and Dispase™ were found to have acceptable purity and stability with thermolysin chosen based on cost. Dose-response experiments correlating Liberase HI mass to islet yields showed that the use of 0.75, 1.5, or 3.0 mg of Liberase HI per mL of solution did not affect human islet yields (4).

Read more here.

 

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