Tissue Dissociation - your first smart move to reproducible results

Tissue Dissociation is used to isolate cells from tissues. The aim is to get the highest number of cells to the best possible quality for further experiments. Critical issue is the quality of used enzymes, purified or crude.

In many labs crude is used which has the big disadvantage of enzymatic contamination. This leads to a low reproducibility and the need to test every new batch for your application.

To overcome these disadvantages we offer only highly purified enzyme systems and guarantee that - once tested - these work every time in the same way. Results will be reproducible and further batch testing is redundant.

Collagenases are enzymes that digest collagen the major fibrous component of animal and human extracellular connective tissue. These enzymes are prepared from C. histolyticum and are often used as crude enzyme preparation. The advantage of bacterial endopeptidases is that these enzymes will digest the insoluble collagen as well as soluble collagen fragments. The disadvantage of using crude collagenase preparations are the contaminating foreign enzyme activities (tryptic, caseinase and clostripain) which prevents reproducibility. Running out of collagenase scientists have to test new batches to find the right one for their application and tissue. Avoiding this batch testing procedure repeatedly would be a big step forward and improve productivity. Highly purified enzyme preparations will make this lot procedure superfluous.

Our partner VitaCyte offers only highly purified enzymes (collagenase and protease) as pure preparation and enzymatic blends. Recently we launched the DE collagenase product family which is characterized by increasing collagenase activities with the same amount of protease activity.

 

CIzyme™  Collagenase  HA         CIzyme™  Collagenase  MA       CIzyme™  Collagenase Gold        DE Collagenase

DE Collagenase consists of increasing amounts of enriched collagenase (> 85% pure) with minimal protease activity add to fixed amount of purified BP Protease. The enriched collagenase has high collagen degradation activity and very low contamination with protease (<0.5%). The following blends are available:

Thermolysin a neutral protease prepared from Bacillus thermoproteolyticus is a thermostable metalloproteinase used for isolation of islets from rodent, canine, monkey and human pancreas.

Clostripain is an endoproteinase Arg-c prepared from  Bclostridium histolyticum and cleaves proteins on the carboxyl peptide bond of arginin. Clostripain is used as a supplement to collagenase in tissue dissociation applications often along with Thermolysin or BP Protease. Each tissue type and primary cell of interest has its own clostripain requirement.

BP Protease (Neutral Protease, Dispase) is prepared from Bacillus polymyxa produced without components of animal orgin. BP Protease was designed to isolate islets, hepatocytes and adipose derived stromal cells (ADSC).

Enzymatic blends are mixtures of purified collagenase and purified proteases designed for special applications to increase reproducibility and lot-to-lot consistency.

We offer tested and pretested blends for rodent islets, adipose derived stem cells and human hepatocytes

  • CIzyme™ AS was developed to isolate adipose derived stromal cells (ADSC) from adipose tissue.
  • CIzyme™ HH was developed to human hepatocytes.
  • CIzyme™ RI was developed to rodent islets.
  • DE Collagenase products are blends of enriched collagenase and purified neutral protease at defined activities to provide a broad spectrum of products with distinct collagenase to neutral protease activities.

Tissue Dissociation

Animal-free Thermolysin (GMP) , , .

GMP grade Thermolysin (Animal component-free) is an aseptically dispensed, lyophilized purified protease from Bacillus thermoproteolyticus. The 6 mg pack size is a thermostable neutral protease that can be used with purified collagenase for isolating rodent islets, cynomolgus monkey, and human pancreata.

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CIzyme AS (GMP) , , .

GMP Grade CIzyme AS is a blend of collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa. CIzyme AS can be used for isolating adipose derived stromal cells from human lipoaspirate.

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CIzyme BP Protease (GMP) , , .

GMP grade BP Protease (Animal component-free) is an aseptically dispensed, lyophilized protein containing purified neutral protease from Bacillus polymyxa. The 1.1 million NP Unit pack size can be used to work with our collagenases to isolate islets, hepatocytes, human epithelial skin cells, and adipose derived stromal cells.

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CIzyme BP Protease (GMP) , , .

GMP grade BP Protease (Animal component-free) is an aseptically dispensed, lyophilized protein containing purified neutral protease from Bacillus polymyxa. The 2.3 million NP Unit pack size can be used to work with our collagenases to isolate islets, hepatocytes, human epithelial skin cells, and adipose derived stromal cells.

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CIzyme Clostripain , , .

CIzyme Clostripain is an aseptically dispensed, lyophilized and purified from Clostidium histolyticum. The 25 mg pack size can be used as a supplement to collagenase in tissue dissociation applications often along with the appropriate neutral protease.

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CIzyme Collagenase MA (GMP) , , .

GMP Grade Collagenase MA is an aseptically filled, lyophilized and purified collagenase from Clostidium histolyticum. 2.5 Million CDA Unit pack size can be used for isolating human hepatocytes with the appropriate neutral protease.

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CIzyme Collagenease HA (GMP) , , .

GMP Grade Collagenase HA is an aseptically prepared, lyophilized and purified Collagenase from Clostridium histolyticum. 2000 Wünsch Unit pack size can be used for isolating islets from human pancreata with the appropriate neutral protease.

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CIzyme Collagenease HA (GMP) , , .

GMP Grade Collagenase HA is an aseptically prepared, lyophilized and purified Collagenase from Clostridium histolyticum. 200 Wünsch Unit pack size can be used for isolating cells from tissue or recovering adherent cells after in vitro culture.

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CIzyme Collagenease MA (GMP) , , .

GMP Grade Collagenase MA is an aseptically filled, lyophilized and purified collagenase from Clostidium histolyticum. 1100 Wünsch Unit pack size can be used for isolating porcine islets with the appropriate neutral protease.

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CIzyme rCollagenase HI , , .

The recombinant Collagenase (rHI) is an aseptically prepared, lyophilized and purified enzyme from engineered E. coli. rHI is manufactured to provide the optimal collagenase activity for recovering islets from human pancreata when combined with purified BP Protease. 

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CIzyme RI , , .

CIzyme RI is an aseptically filled, lyophilized mixture of 60% purified C1 and 40% purified C2 collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa. CIzyme RI was developed using C57BL/6 mice and can be used for isolating rodent islets.

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Collagenase Gold , , .

The Collagenase Gold is a low protease, enriched collagenase product from Clostridium histolyticum that can be used for isolating cells from tissue or recovering adherent cells after in vitro culture. This product can be used for isolating human islets with the appropriate neutral protease.

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Collagenase Gold , , .

The Collagenase Gold is a low protease, enriched collagenase product from Clostridium histolyticum that can be used for isolating cells from tissue or recovering adherent cells after in vitro culture. This product can be used for isolating human islets with the appropriate neutral protease.

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Collagenase Gold Plus (GMP) , , .

#human islet isolation from healthy tissues with the appropriate neutral protease.

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DE Collagenase 10 , , .

DE Collagenase 10 can be used for isolating porcine hepatocytes and rodent hepatocytes.

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DE Collagenase 100 , , .

DE Collagenase 100 can be used for isolating porcine hepatocytes and rodent hepatocytes.

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DE Collagenase 20 , , .

DE Collagenase 20 can be used for isolating rat hepatocytes, human foreskin fibroblasts, human foreskin keratinocytes, and human glioblastomas.

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DE Collagenase 200 , , .

DE Collagenase 200 can be used for isolating rat hepatocytes, human foreskin fibroblasts, human foreskin keratinocytes, and human glioblastomas.

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DE Collagenase 40 , , .

DE Collagenase 40 can be used for rodent islets and rodent dendritic cells.  

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DE Collagenase 400 , , .

DE Collagenase 400 can be used for rodent islets and rodent dendritic cells.  

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DE Collagenase 60 , , .

DE Collagenase 60 can be used for isolating rodent islets and rodent pancreatic cells.

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DE Collagenase 600 , , .

DE Collagenase 600 can be used for isolating rodent islets and rodent pancreatic cells.

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DE Collagenase 80 , , .

DE Collagenase 80 can be used for isolating porcine kidney epithelial cells, rat cardiomyocytes, rat fibroblasts, and rodent dendritic cells.

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DE Collagenase 800 , , .

DE Collagenase 800 can be used for isolating porcine kidney epithelial cells, rat cardiomyocytes, rat fibroblasts, and rodent dendritic cells.

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DE Collagenase Optimization kit , , .

VitaCyte´s optimization kit provides one 100 mg pack size from each of the five formulations comprising the full spectrum of DE Collagenase products. Apply each sample to the cells or organs you’re using to see which performs best. DE Collagenase performs consistently across lots, so you’ll never have to lot test again.

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