The Stemgent #mRNA Reprogramming Kit, in combination with the microRNA Booster Kit (00-0073), is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human #iPS cells. Generate iPS cells in less than 16 days and expand new lines in culture in as little as 3 weeks as compared to lentivirus, Sendai virus, and other methods, that can take over 20 weeks to confirm that viral remnants no longer remain. This system completely eliminates the concern of virus bio-containment and safety issues.
Fully tested and validated, the Stemgent mRNA Reprogramming Kit includes five mRNA reprogramming factors, a nuclear GF P marker for monitoring transfection efficiency, the Stemgent Pluriton™ Reprogramming Medium, B18R protein, and a reproducible, optimized protocol. This kit has been validated to successfully reprogram human BJ fibroblasts as a control cell line. The Stemgent mRNA Reprogramming Kit enables researchers greater ease-of-use with the ability to simply regulate the timing of protein expression levels and have stoichiometric control of individual reprogramming factors.
• Non-integrating — no risk of genomic integration
• Faster reprogramming, colonies can emerge appear in as early as 12 days
• Stoichiometric control of individual reprogramming factors
• Eliminates need for multi-step passaging and screening of colonies
• Validated protocol using normal and diseased cells
• Hands-on training and on-site support