Archive for Category: General

Showing 71 – 80 of 104 results

  • Differential Hypoxic Response of Human Choroidal and Retinal Endothelial cells Proposes Tissue Heterogeneity of Ocular Angiogenesis

    Human CEC and REC were isolated by positive selection and were cultured. Characterization was performed by immunostaining for endothelial cell (EC)-specific markers. Total RNA and protein were extracted from normoxic or hypoxic CEC and REC cultures. Quantitative polymerase chain reaction (PCR) arrays were used to comparatively analyse 133 genes between CEC and REC, and the expression differences were calculated by ΔΔCt method. A total of 57 angiogenesis-related protein expression differences were...
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  • GeneAll: New References

    A disulphide-linked Heterodimer of TWIK-1 and TREK-1 mediates passive conductance in astrocytes TWIK-1 is a member of the two-pore domain K þ (K2P) channel family that plays an essential part in the regulation of resting membrane potential and cellular excitability. The physiological role of TWIK-1 has remained enigmatic because functional expression of TWIK-1 channels is elusive. Here we report that native TWIK-1 forms a functional channel at the plasma membrane of astrocytes. A search for TWIK-1-binding...
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  • International Stem Cell Guidelines Revised, Expanded

    Widely recognized stem cell research guidelines now address controversial topics such as human gene editing. The decision by the International Society for Stem Cell Research to recommend that stem cell oversight bodies should review all embryo research comes from a recognition of rapidly advancing technologies that could make unprecedented advances in understanding human disease but also have ethical implications. “We in the scientific community have to be vigilant—and also transparent—about...
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  • S-stamp Marking Technique helps prevent Inverted Graft Implantation in DMEK

    Endothelial cell loss, re-bubbling and complication rates were similar in stamped and unstamped graft groups. A novel S-stamp marking method eliminated upside-down graft implantation in Descemet’s membrane endothelial keratoplasty, according to a study. The technique may help surgeons perfect the DMEK technique and avoid complications, according to Peter B. Veldman, MD, the corresponding author. To read more go to healio.
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  • Gene Editing Technique Improves Vision in Rats with Inherited Blindness

    A Cedars-Sinai study has revealed that a research team at the Cedars-Sinai Board of Governors Regenerative Medicine Institute has discovered a new technique that has the potential to treat inherited diseases by removing genetic defects has been shown for the first time to hinder retinal degeneration in rats with a type of inherited blindness. The focus of the researchers was on inherited retinitis pigmentosa – a degenerative eye disease with no known cure that can lead to blindness. Â A technique...
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  • Researchers Create Micro Heart Muscle From Stem Cells

    The medical research field has been looking into creating better solutions for delicate yet complex procedures that require utmost precision when dealing with heart problems and stem cell therapy is one of the possible solutions for treating heart failure. Luckily, Gladstone Institutes, whose mission is to overcome major unsolved diseases, has just given coronary disease researchers an excellent tool to advance methods and medicines for heart problems with its latest breakthrough. To read more...
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  • Damaging Consequences of Zika Virus Infection in Human Minibrains

    Brazilian researchers from the D’Or Institute for Research and Education (IDOR) and Federal University of Rio de Janeiro (UFRJ) have demonstrated the harmful effects of ZIKA virus (ZIKV) in human neural stem cells, neurospheres and brain organoids. Since ZIKV has been gradually established as a direct cause of central nervous system malformations, this study help to elucidate the etiological nature of the recently increasing number of microcephaly cases in Brazil. To read more, please click h...
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  • Using Gene Editing to Delete HIV from Human T Cells

    Researchers at Temple University used a CRISPR gene editing technique to remove HIV from the DNA of human cells grown in culture. Their study (“Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing”) is published In Scientific Reports. According to senior investigator on the new study, Kamel Khalili, Ph.D., Laura H. Carnell Professor and Chair of the Department of Neuroscience, Director of the Center for Neurovirology, and Director of the Comprehensive NeuroAIDS...
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  • Removing Epigenetic Post-it Notes Returns Stem Cells to Unprimed State

    A drug originally intended to treat leukemia has an unexpected power. It can reverse stem cell development, converting primed pluripotent stem cells to naïve pluripotent stem cells. The drug, called MM-401, effectively removes epigenetic markers from histones, depriving the cell’s DNA-reading machinery of indications of where to start reading. Stripped of its accumulated “Post-it notes,” the DNA instruction manual is like new. It lacks any indications that any sections should receive any special...
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  • Co-Culture of Aortic Endothelial & Smooth Muscle Cells

    A novel role of endothelium in activation of latent pro-membrane type 1 MMP and pro-MMP-2 in rat aorta. Aims Aortic stiffness is an independent risk factor for progression of cardiovascular diseases. Degradation of elastic fibres in aorta due to angiotensin II (ANGII)-stimulated overactivation of latent membrane type 1 matrix metalloproteinase (MT1MMP) and matrix metalloproteinase-2 (MMP2) is regarded to represent an important cause of aortic stiffness. Therefore, clarification of the causal mechanisms...
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